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An Introduction to Single Molecule Biophysics

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An Introduction to Single Molecule Biophysics

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An Introduction to Single Molecule Biophysics
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Description

This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.

Author Biography

Yuri L. Lyubchenko is Professor of Pharmaceutical Sciences at the University of Nebraska Medical Center, Omaha, NE, USA. His research focuses on understanding fundamental mechanisms underlying health and disease, which are key to developing new and more effective diagnostics and medications. This primarily basic research allows him not only identify new drug targets for small molecule drugs, it also leads to development of the nanotools and methods to discover novel approaches for diagnostic, treatment, and disease prevention, and to more rapidly determine their efficacy at the molecular level.
Release date Australia
June 28th, 2018
Contributor
Edited by Yuri L. Lyubchenko
Country of Publication
United Kingdom
Illustrations
2 Tables, black and white; 99 Illustrations, color; 5 Illustrations, black and white
Imprint
CRC Press
Pages
240
ISBN-13
9781138615960
Product ID
28249264

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