Bacopa monnieri (family: Plantaginaceae) a small, common, amphibious plant growing in marshy areas throughout the Indian subcontinent. The entire plant constitutes well known drug Brahmi. It is astringent, bitter, cooling and is reported to improve the intellect. The drug is used in the number of ayurvedic preparation such as Brahmighritam and Brahmirasayanam. Tissue culture methods offer an alternative means of plant vegetative propagation. The rapid progress in the area of crop biotechnology is mainly because of the development of efficient regeneration and suitable Agrobacterium-mediated transformation protocols for different crop species. Similar success can be achieved in medicinal plants by developing efficient regeneration and Agrobacterium-mediated transformation protocols, which in turn could be used for the enhancement of their secondary metabolite content.