This guide of recombinant FVIII production brings the power of manipulating and customizing genes to obtain ideal function of proteins. You will learn What is coagulation cascade and How FVIII works together with other factors in this cascade, what is the primary structure of FVII and where is spotlight of the gene and sequences that interact with other proteins, how to work with plasmids and generally cloning purpose, how to work with cell lines and how to optimize transfection process, brief discussions about bioinformatics essentials and being familiar with some online bioinformatic software and databases. All the protocols have been thoroughly tested in the author's own laboratory.