Non-Fiction Books:

DNA Cloning: A Hands-on Approach

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Hardback
$407.99
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Description

This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail. 

Author Biography:

Seok-Yong Choi, Department of Biomedical Sciences, Chonnam National University College of Medicine, Gwangju, Republic of Korea Hyunju Ro, Department of Biological Sciences, Chungnam National University, Daejeon, Republic of Korea Hankuil Yi, Department of Biological Sciences, Chungnam National University, Daejeon, Republic of Korea
Release date Australia
May 14th, 2019
Audience
  • Professional & Vocational
Edition
1st ed. 2019
Illustrations
70 Illustrations, color; 23 Illustrations, black and white; XIII, 131 p. 93 illus., 70 illus. in color.
Pages
131
ISBN-13
9789402416602
Product ID
28816318

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