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Gene Cloning and DNA Analysis

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Gene Cloning and DNA Analysis

An Introduction



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Gene Cloning and DNA Analysis: An Introduction by T.A. Brown
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Known world-wide as the standard introductory text to thisimportant and exciting area, the sixth edition of Gene Cloningand DNA Analysis addresses new and growing areas of researchwhilst retaining the philosophy of the previous editions. Assumingthe reader has little prior knowledge of the subject, itsimportance, the principles of the techniques used and theirapplications are all carefully laid out, with over 250 clearlypresented four-colour illustrations.

In addition to a number of informative changes to the textthroughout the book, the final four chapters have beensignificantly updated and extended to reflect the striking advancesmade in recent years in the applications of gene cloning and DNAanalysis in biotechnology.

Gene Cloning and DNA Analysis remains an essentialintroductory text to a wide range of biological sciences students;including genetics and genomics, molecular biology, biochemistry,immunology and applied biology. It is also a perfect introductorytext for any professional needing to learn the basics of thesubject. All libraries in universities where medical, life andbiological sciences are studied and taught should have copiesavailable on their shelves.

" the book content is elegantly illustrated and wellorganized in clear-cut chapters and subsections there is aFurther Reading section after each chapter that contains severalkey references What is extremely useful, almost everyreference is furnished with the short but distinct author'sremark." Journal of Heredity, 2007 (on the previousedition)

Table of Contents

Preface to the Sixth Edition.

Part 1: The Basic Principles of Gene Cloning and DNAAnalysis.

1 Why Gene Cloning and DNA Analysis are Important.

1.1 The early development of genetics.

1.2 The advent of gene cloning and the polymerase chainreaction.

1.3 What is gene cloning?

1.4 What is PCR?

1.5 Why gene cloning and PCR are so important.

1.6 How to find your way through this book.

2 Vectors for Gene Cloning: Plasmids andBacteriophages.

2.1 Plasmids.

2.2 Bacteriophages.

3 Purification of DNA from Living Cells.

3.1 Preparation of total cell DNA.

3.2 Preparation of plasmid DNA.

3.3 Preparation of bacteriophage DNA.

4 Manipulation of Purified DNA.

4.1 The range of DNA manipulative enzymes.

4.2 Enzymes for cutting DNA restrictionendonucleases.

4.3 Ligation joining DNA molecules together.

5 Introduction of DNA into Living Cells.

5.1 Transformation the uptake of DNA by bacterialcells.

5.2 Identification of recombinants.

5.3 Introduction of phage DNA into bacterial cells.

5.4 Identification of recombinant phages.

5.5 Introduction of DNA into non-bacterial cells.

6 Cloning Vectors for E. coli.

6.1 Cloning vectors based on E. coli plasmids.

6.2 Cloning vectors based on M13 bacteriophage.

6.3 Cloning vectors based on l bacteriophage.

6.4 and other high capacity vectors enable genomiclibraries to be constructed.

6.5 Vectors for other bacteria.

7 Cloning Vectors for Eukaryotes.

7.1 Vectors for yeast and other fungi.

7.2 Cloning vectors for higher plants.

7.3 Cloning vectors for animals.

8 How to Obtain a Clone of a Specific Gene.

8.1 The problem of selection.

8.2 Direct selection.

8.3 Identification of a clone from a gene library.

8.4 Methods for clone identification.

9 The Polymerase Chain Reaction.

9.1 The polymerase chain reaction in outline.

9.2 PCR in more detail.

9.3 After the PCR: studying PCR products.

9.4 Real time PCR enables the amount of starting material to bequantified.

Part 2: The Applications of Gene Cloning and DNA Analysis inResearch.

10 Sequencing Genes and Genomes.

10.1 The methodology for DNA sequencing.

10.2 How to sequence a genome.

11 Studying Gene Expression and Function.

11.1 Studying the RNA transcript of a gene.

11.2 Studying the regulation of gene expression.

11.3 Identifying and studying the translation product of acloned gene.

12 Studying Genomes.

12.1 Genome annotation.

12.2 Studies of the transcriptome and proteome.

Part 3: The Applications of Gene Cloning and DNAAnalysis in Biotechnology.

13 Production of Protein from Cloned Genes.

13.1 Special vectors for expression of foreign genes in E.coli.

13.2 General problems with the production of recombinant proteinin E. coli.

13.3 Production of recombinant protein by eukaryotic cells.

14 Gene Cloning and DNA Analysis in Medicine.

14.1 Production of recombinant pharmaceuticals.

14.2 Identification of genes responsible for human diseases.

14.3 Gene therapy.

15 Gene Cloning and DNA Analysis in Agriculture.

15.1 The gene addition approach to plant geneticengineering.

15.2 Gene subtraction.

15.3 Problems with genetically modified plants.

16 Gene Cloning and DNA Analysis in Forensic Science andArchaeology.

16.1 DNA analysis in the identification of crime suspects.

16.2 Studying kinship by DNA profiling.

16.3 Sex identification by DNA analysis.

16.4 Archaeogenetics using DNA to study humanprehistory.



Author Biography

Terry Brown, University of Manchester, Faculty of Life Sciences, UK

Release date Australia
March 26th, 2010
Country of Publication
United Kingdom
6th Revised edition
Wiley-Blackwell (an imprint of John Wiley & Sons Ltd)
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