Non-Fiction Books:

Immunohistochemistry: Basics and Methods

Format

Hardback

Customer rating

Click to share your rating 0 ratings (0.0/5.0 average) Thanks for your vote!

Share this product

Immunohistochemistry: Basics and Methods by Igor B. Buchwalow
Save $213.00
$328.99 was $541.99
or 4 payments of $82.25 with Learn more
In stock with supplier

The item is brand new and in-stock in with one of our preferred suppliers. The item will ship from the Mighty Ape warehouse within the timeframe shown below.

Usually ships within 10-14 days

Availability

Delivering to:

Estimated arrival:

  • Around 5-11 September using Express Delivery
    Mighty Ape can deliver this product within 1-2 business days (usually overnight) to urban centres across Australia, and some remote areas. Learn more
  • Around 6-13 September using standard courier delivery

Description

Combiningtwodifferentscienti?cdisciplines-morphologyandimmunochemistry- immunohistochemistryhasdevelopedasanimportantinstrumentinresearchand clinicalpathology. A basicunderstandingofunderlying principlesandpotential problemsisunavoidableifyouwanttobesuccessfulinyouruseofimmunohis- chemistry,aswellasingettingyourpaperspublishedandyourresearchgrants funded. Whilemanyexcellenttextsandmonographsexistwhichcovervariousaspects ofimmunohistochemistry,thelackofaconcisecomprehensiveguidetousing thesemethodswasamajormotivationforwritingthisbook. Ourintentionwasto createaneasy-to-readandfocusedresourcebasedonstate-of-the-artinformation forabroadaudiencerangingfromstudentsandtechnicalassistantstoexperienced researchers. Thishandbookhasaconciseformat,withprotocolsandinstructionsfor methodsimmediatelyfollowingtheshortintroductorytheoreticalmaterialineach chapter. BeingconsciousofthegrowingroleofInternetasaninformationsource, wehavefounditreasonableinmanycasestosubstitutecitingbooksandjournal publications with corresponding Internet websites. Where possible, commercial sourcesofreagents,kits,andequipmentarelistedthroughoutthetextinsteadof inaseparateindex. Thougheachchapterissmallandintroductory,thishandbook itself is self-suf?cient and provides a comprehensive look at the principles of immunohistochemistry. For readers wanting further depth of knowledge, each chapterisbackedupbyashortlistofcarefullyselectedoriginalarticles. Duringthelastdecade,pioneeringeffortsofhistochemistshaveledtoan- menseimprovementinthereagentsandprotocols. Theresearcherisurgedalwaysto determinethereasonforeverymethodandstepbeforedoingit. Thishandbookis intendedtohelpreaderstoavoidtroublesinthechoiceofanadequatemethod,which happenswhenusingstandardtextbooks. Forthishandbook,wecarefullyselected establishedmethodsandeasy-to-adoptprotocols,payingattentiontomoderndev- opmentsinimmunohistochemistry,suchasantigenretrieval,signalampli?cation, the use of epitope tags in immunohistochemistry, multiple immunolabeling or diagnosticimmunohistochemistry. Eachofthemethodsdescribedinthishandbook v vi Preface was provedby the authors; many of these methods are routinely used in daily practiceintheirinstitute. Allthepracticalmethodsadvocatedareclearlydescribed, withaccompanyingtables,andtheresultsobtainableareillustratedwithcolour micrographs. Acknowledgements We thank Vera Samoilova forthe perfect technical assistance and other colleaguesfromtheMunsterUniversityClinicforsharingprobesandreagents. IgorB. BuchwalowandWernerBocker Munster Contents 1 AntibodiesforImmunohistochemistry ...1 1. 1 StructureofAntibodies ...2 1. 2 PolyclonalAntibodies ...4 1. 3 MouseMonoclonalAntibodies ...4 1. 4 RabbitMonoclonalAntibodies ...5 1. 5 ProteinAandProteinGinImmunohistochemistry ...7 References ...8 2 AntibodyLabelingandtheChoiceoftheLabel ...9 2. 1 CovalentLabelingofAntibodies ...9 2. 2 Non-CovalentLabelingofPrimaryAntibodieswith LabeledFabFragments ...10 2. 3 EnzymeLabelsforLightMicroscopy ...13 2. 4 FluorophoreLabelsforFluorescenceMicroscopy ...15 2. 5 ColloidalGoldLabelsforElectronMicroscopy ...16 References ...17 3 ProbesProcessinginImmunohistochemistry ...19 3. 1 FixationinImmunohistochemistry ...19 3. 1. 1 FixationinAlcoholsandAcetone ...20 3. 1. 2 FixationinFormaldehyde ...20 3. 1. 3 EffectofFormaldehydeFixationon Antigen-nster Contents 1 AntibodiesforImmunohistochemistry ...1 1. 1 StructureofAntibodies ...2 1. 2 PolyclonalAntibodies ...4 1. 3 MouseMonoclonalAntibodies ...4 1. 4 RabbitMonoclonalAntibodies ...5 1. 5 ProteinAandProteinGinImmunohistochemistry ...7 References ...8 2 AntibodyLabelingandtheChoiceoftheLabel ...9 2. 1 CovalentLabelingofAntibodies ...9 2. 2 Non-CovalentLabelingofPrimaryAntibodieswith LabeledFabFragments ...10 2. 3 EnzymeLabelsforLightMicroscopy ...13 2. 4 FluorophoreLabelsforFluorescenceMicroscopy ...15 2. 5 ColloidalGoldLabelsforElectronMicroscopy ...16 References ...17 3 ProbesProcessinginImmunohistochemistry ...19 3. 1 FixationinImmunohistochemistry ...19 3. 1. 1 FixationinAlcoholsandAcetone ...20 3. 1. 2 FixationinFormaldehyde ...20 3. 1. 3 EffectofFormaldehydeFixationon Antigen- immunohistochemistryhasdevelopedasanimportantinstrumentinresearchand clinicalpathology. A basicunderstandingofunderlying principlesandpotential problemsisunavoidableifyouwanttobesuccessfulinyouruseofimmunohis- chemistry,aswellasingettingyourpaperspublishedandyourresearchgrants funded. Whilemanyexcellenttextsandmonographsexistwhichcovervariousaspects ofimmunohistochemistry,thelackofaconcisecomprehensiveguidetousing thesemethodswasamajormotivationforwritingthisbook. Ourintentionwasto createaneasy-to-readandfocusedresourcebasedonstate-of-the-artinformation forabroadaudiencerangingfromstudentsandtechnicalassistantstoexperienced researchers. Thishandbookhasaconciseformat,withprotocolsandinstructionsfor methodsimmediatelyfollowingtheshortintroductorytheoreticalmaterialineach chapter. BeingconsciousofthegrowingroleofInternetasaninformationsource, wehavefounditreasonableinmanycasestosubstitutecitingbooksandjournal publications with corresponding Internet websites. Where possible, commercial sourcesofreagents,kits,andequipmentarelistedthroughoutthetextinsteadof inaseparateindex. Thougheachchapterissmallandintroductory,thishandbook itself is self-suf?cient and provides a comprehensive look at the principles of immunohistochemistry. For readers wanting further depth of knowledge, each chapterisbackedupbyashortlistofcarefullyselectedoriginalarticles. Duringthelastdecade,pioneeringeffortsofhistochemistshaveledtoan- menseimprovementinthereagentsandprotocols. Theresearcherisurgedalwaysto determinethereasonforeverymethodandstepbeforedoingit. Thishandbookis intendedtohelpreaderstoavoidtroublesinthechoiceofanadequatemethod,which happenswhenusingstandardtextbooks. Forthishandbook,wecarefullyselected establishedmethodsandeasy-to-adoptprotocols,payingattentiontomoderndev- opmentsinimmunohistochemistry,suchasantigenretrieval,signalampli?cation, the use of epitope tags in immunohistochemistry, multiple immunolabeling or diagnosticimmunohistochemistry. Eachofthemethodsdescribedinthishandbook v vi Preface was provedby the authors; many of these methods are routinely used in daily practiceintheirinstitute. Allthepracticalmethodsadvocatedareclearlydescribed, withaccompanyingtables,andtheresultsobtainableareillustratedwithcolour micrographs. Acknowledgements We thank Vera Samoilova forthe perfect technical assistance and other colleaguesfromtheMunsterUniversityClinicforsharingprobesandreagents. IgorB. BuchwalowandWernerBocker Munster Contents 1 AntibodiesforImmunohistochemistry ...1 1. 1 StructureofAntibodies ...2 1. 2 PolyclonalAntibodies ...4 1. 3 MouseMonoclonalAntibodies ...4 1. 4 RabbitMonoclonalAntibodies ...5 1. 5 ProteinAandProteinGinImmunohistochemistry ...7 References ...8 2 AntibodyLabelingandtheChoiceoftheLabel ...9 2. 1 CovalentLabelingofAntibodies ...9 2. 2 Non-CovalentLabelingofPrimaryAntibodieswith LabeledFabFragments ...10 2. 3 EnzymeLabelsforLightMicroscopy ...13 2. 4 FluorophoreLabelsforFluorescenceMicroscopy ...15 2. 5 ColloidalGoldLabelsforElectronMicroscopy ...16 References ...17 3 ProbesProcessinginImmunohistochemistry ...19 3. 1 FixationinImmunohistochemistry ...19 3. 1. 1 FixationinAlcoholsandAcetone ...20 3. 1. 2 FixationinFormaldehyde ...20 3. 1. 3 EffectofFormaldehydeFixationon Antigen-AntibodyBinding ...21 3. 2 Paraf?nSectionsforImmunohistochemicalAnalysis ...22 3. 2. 1 EmbeddingandCutting ...22 3. 2. 2 MountingParaf?nSectionsontoSlides ...23 3. 3 CryosectionsforImmunohistochemicalAnalysis ...24 3. 4 BuffersforWashingandAntibodyDilution ...25 3. 5 MountingFollowingImmunohistochemicalStaining ...27 3. 6 StorageFollowingImmunohistochemicalStaining ...28 References ...28 vii viii Contents 4 WorkingwithAntibodies ...31 4. 1 DirectImmunostainingMethod ...31 4. 2 IndirectImmunostainingMethod ...33 4. 3 TheChoiceofAntibodies ...35 4. 3. 1 TheChoiceofPrimaryAntibodies ...35 4. 3. 2 TheChoiceofSecondaryAntibodies ...
Release date Australia
February 4th, 2010
Country of Publication
Germany
Edition
2010 ed.
Illustrations
X, 153 p.
Imprint
Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Pages
153
Dimensions
155x235x15
ISBN-13
9783642046087
Product ID
3682680

Customer reviews

Nobody has reviewed this product yet. You could be the first!

Write a Review

Marketplace listings

There are no Marketplace listings available for this product currently.
Already own it? Create a free listing and pay just 9% commission when it sells!

Sell Yours Here

Help & options

  • If you think we've made a mistake or omitted details, please send us your feedback. Send Feedback
  • If you have a question or problem with this product, visit our Help section. Get Help
  • Seen a lower price for this product elsewhere? We'll do our best to beat it. Request a better price
Filed under...