Non-Fiction Books:

Low-Cost Methods for Molecular Characterization of Mutant Plants

Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

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Low-Cost Methods for Molecular Characterization of Mutant Plants by Bradley J. Till
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Description

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Release date Australia
October 17th, 2016
Country of Publication
Switzerland
Edition
Softcover reprint of the original 1st ed. 2015
Illustrations
3 Illustrations, color; 6 Illustrations, black and white; X, 35 p. 9 illus., 3 illus. in color.
Imprint
Springer International Publishing AG
Pages
35
Dimensions
155x235x3
ISBN-13
9783319386676
Product ID
26207324

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