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Mass Spectrometry of Glycoproteins

Methods and Protocols
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Description

 Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol  is  an essential resource for those who work at the interface of glycobiology and mass spectrometry.
Release date Australia
August 23rd, 2016
Contributors
  • Edited by Jennifer J. Kohler
  • Edited by Steven M. Patrie
Pages
339
Edition
Softcover reprint of the original 1st ed. 2013
Audience
  • Professional & Vocational
Illustrations
XI, 339 p.
Dimensions
178x254x19
ISBN-13
9781493962037
Product ID
25507599

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Proteins