Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In "Viral Applications of Green Fluorescent Protein: Methods and Protocols", leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful "Methods in Molecular Biology" series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.The accompanying CD-ROM contains color figures and multi-colored video sequences provided by leading viral researchers. Cutting-edge and easy to use, "Viral Applications of Green Fluorescent Protein: Methods and Protocols" supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.

Table of Contents

1 Using Fluorescent Proteins to Study Poxvirus Morphogenesis Brian M. Ward 2 Application of Reporter Gene Imaging for Studying Human Embryonic Stem Cells in Living Subjects Kazim H. Narsinh, Feng Cao, and Joseph C. Wu 3 Use of a GFP-PML-expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection Tomonori Ueno and Kiyoko Ogawa-Goto 4 Dual-Color Imaging of Tumor Angiogenesis Robert M. Hoffman 5 Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System Alan R. Harvey, Erich Ehlert, Joris de Wit, Eleanor S. Drummond, Margaret A. Pollett, Marc Ruitenberg, Giles W. Plant, Joost Verhaagen, and Christiaan Levelt 6 Expression of EGFP by Adenovirus-mediated Gene Transfer in the Central Nervous System Ryohei Tomioka 7 Adenovirus-mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes Andreas Rinne, Christoph Littwitz, Kirsten Bender, Marie-Cecile Kienitz, and Lutz Pott 8 Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry Michael Grusch, Annemarie Losert, Andreas Lackner, Alev Deli, Irene Herbacek, and Klaus Holzmann 9 Production of Multicistronic HIV-1 Based Lentiviral Vectors Nachimuthu Chinnasamy, James Shaffer, and Dhanalakshmi Chinnasamy 10 Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps Claude Bagnis, Pascal Bailly, and Sylvie Chapel-Fernandes 11 Use of a Macrophage-tropic GFP-tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs Amanda M. Brown 12 GFP-Lentiviral Vectors Targeting for NeuroAIDS Yuanan Lu 13 Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery Aaron M. Jasnow, Donald G. Rainnie, Kimberly A. Maguschak, Jasmeer P. Chhatwal, and Kerry J. Ressler 14 Selective Protein Expression Within the CNS Using Hybrid Lentivirus Fredrik Gussing, Ingrid van Marion, Igor Ralets, and Cecilia Lundberg 15 Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein Bruce W. Banfield and Gregory A. Bird 16 The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication Travis J. Taylor and David M. Knipe 17 Modified Bovine Herpesvirus 1 for Protein Secretion Gunther M. Keil 18 Identification of Viral Peptide Fragments for Vaccine Development Zhanglin Lin, Shuang Li, and Yong Chen 19 Use of GFP to Investigate Expression of Plant-derived Vaccines Hong-Ye Li and Mee-Len Chye 20 Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions Sophie Haupt, Angelika Ziegler, Graham Cowan, and Lesley Torrance 21 Sendai Virus for Cancer Immunotherapy Yasuji Ueda, Mamoru Hasegawa, and Yoshikazu Yonemitsu 22 Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells Qigui Yu, Ningjie Hu, and Mario Ostrowski 23 Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity Misako Yoneda, Kentaro Fujita, Hiroki Sato, and Chieko Kai 24 Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells Chien-Tai Tsai, Ching-Kuang Chuang, and Yu-Chen Hu