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Autoradiography is the localization within a solid specimen of a radiolabel by placing the specimen against a layer of detector material. Many types of specimen can be studied, from whole organisms to ultra-thin cryosections. Autoradiography can supply information at the macroscopic, microscopic and ultrastructural levels, and its technique is therefore of interest to biological students in a variety of research areas. This handbook provides an introduction to the theory and major techniques of autoradiography in a simplified but comprehensive manner. The author describes the rationale behind the methods, and considers the appraisal of autoradiographs.
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