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2-D Proteome Analysis Protocols on Hardback
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2-D Proteome Analysis Protocols

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Description

A step-by-step tour through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. 2-D Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions.
Release date Australia
September 24th, 1998
Audiences
  • Postgraduate, Research & Scholarly
  • Professional & Vocational
  • Undergraduate
Contributor
  • Edited by Andrew J. Link
Illustrations
XVII, 601 p.
Pages
601
Dimensions
155x235x38
ISBN-13
9780896035249
Product ID
5261525

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Books >
Non-Fiction >
Maths & Science >
Biology, life sciences >
Life sciences: general issues >
Genetics
Books >
Non-Fiction >
Maths & Science >
Biology, life sciences >
Biochemistry
Books >
Non-Fiction >
Maths & Science >
Biology, life sciences >
Biochemistry >
Proteins
Books >
Non-Fiction >
Maths & Science >
Biology, life sciences >
Molecular biology