In the spirit of Gladiator and Xena-Warrior Princess, Amazons and Gladiators is an epic tale of good verus evil. In a remote village at the edge of the Ancient Roman Empire, a young girl is enslaved and her family butchered by the sadistic Roman General Crassius (Patrick Bergin). As a beautiful young woman, Serena is a reluctant slave-dancer, but after stabbing a depraved Roman senator in self-defence she is forced to escape from her master in the company of a fearsome Amazonian warrior (Jennifer Rubin).

Under the protection of the Amazons, Serena learns the art of mortal combat and, driven by her hatred of Crassius, becomes one of the most accomplished and deadly fighters in the tribe. Unbeknown to Serena, Crassius is now stationed as Governor to a nearby province and has taken her estranged sister as a willing slave and mistress. Captured again by the Romans after an abortive attempt on Crassius's life, Serena finds herself in a savage gladatorial arena, pitted against impossible odds, but resolved to avenge her parents death at any cost.