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Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis by Fangjun Wang
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Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis

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Description

In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.
Release date Australia
August 27th, 2016
Author
Pages
94
Edition
Softcover reprint of the original 1st ed. 2014
Audience
  • Professional & Vocational
Illustrations
23 Illustrations, color; 19 Illustrations, black and white; XIII, 94 p. 42 illus., 23 illus. in color.
Dimensions
155x235x6
ISBN-13
9783662524329
Product ID
25774497

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Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis on Hardback by Fangjun Wang
$260.99
Applications of Monolithic Column and Isotope Dimethylation Labeling...
by Fangjun Wang ~ Hardback
Available - Usually ships in 3-4 weeks

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