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A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry by Xiaoshi Wang
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A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry

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Description

In this thesis, Xiaoshi Wang investigates the function and mechanism of a newly discovered heme-thiolate peroxygenase, AaeAPO. This enzyme class comes from Agrocybe aegerita and is used in the conversion of inert hydrocarbons to alcohols. Xiaoshi's work focuses on an extracellular P450 enzyme which is not limited in its stability and lack of solubility and therefore is relevant for widespread industrial use. The author demonstrates that the peroxygenase catalyzes a wide range of reactions. In some cases the author even describes very difficult transformations in molecules that are highly inert. Her detailed investigations provide a mechanistic framework for how the peroxygenase catalyzes such a large number of reactions. A major highlight of this thesis is the identification of key short-lived intermediates in the catalytic cycle of the peroxygenase, using rapid kinetic and spectroscopic methods, as well as the elucidation of the thermodynamic properties of these high-energy intermediates. This work adds new insight into an important class of enzymes.

Author Biography:

Xiaoshi Wang received her B.Sc. in chemistry from Nanjing University, China in 2007. She obtained her Ph.D. in chemistry from Chemistry Department of Princeton University in August 2013. Her major research project in Prof. John T. Groves group is function and mechanistic investigation on C-H oxygenation reactions catalyzed by heme-thiolate enzymes. Subsequently, she became a post-doc fellow working with Prof. Benjamin Garcia at Perelman School of Medicine, University of Pennsylvania, focusing on studying chromatin protein PTMs during epigenetic processes.
Release date Australia
August 23rd, 2016
Author
Audience
  • Professional & Vocational
Edition
Softcover reprint of the original 1st ed. 2016
Illustrations
XXXII, 130 p.
Pages
130
ISBN-13
9783319354286
Product ID
28500573

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A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry on Hardback by Xiaoshi Wang
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A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for...
by Xiaoshi Wang ~ Hardback
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