All the biological parameters done from fresh cows' urine of different stages respectively Calves, Pregnant cows, Milking cows. From every stage four cows were selected and urine samples taken at week intervals in early morning time.The pH of cow urine in each stage cow was alkaline with exception in C1 at week-3 and in P2 at week -1 where the pH was 6.93 and 6.97 respectively and EC was highest in calves. Urea and Phenol and amino acid contents found highest in pregnant followed by milch cows than calves. Amylase activity and uric acid were significantly highest in urine of milking cows. Total nitrogen content was maximum in pregnant cows while it was true for sulfur content for calves. The calves showed highest mean value of sodium content while the pregnant cows showed the highest content of potassium. Calcium and magnesium were maximum in urine of pregnant cows. Manganese was not varied from individual cows except few.