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Directed Evolution

Methods and Protocols
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Description

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.
Release date Australia
June 23rd, 2023
Audience
  • Professional & Vocational
Contributors
  • Edited by Andrew Currin
  • Edited by Neil Swainston
Edition
1st ed. 2022
Illustrations
58 Illustrations, color; 5 Illustrations, black and white; XI, 278 p. 63 illus., 58 illus. in color.
Pages
278
ISBN-13
9781071621547
Product ID
36803905

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