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Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions by Ang Yan Shan
Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions by Ang Yan Shan
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Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions

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Description

This book provides essential insights into designing a localized DNA circuit to promote the rate of desired hybridization reactions over undesired leak reactions in the bulk solution. The area of dynamic DNA nanotechnology, or DNA circuits, holds great promise as a highly programmable toolbox that can be used in various applications, including molecular computing and biomolecular detection. However, a key bottleneck is the recurring issue of circuit leakage. The  assembly of the localized circuit is dynamically driven by the recognition of biomolecules – a different approach from most methods, which are based on a static DNA origami assembly. The design guidelines for individual reaction modules presented here, which focus on minimizing circuit leakage, are established through NUPACK simulation and tested experimentally – which  will be useful for researchers interested in adapting the concepts for other contexts. In the closing section, the design concepts are successfully applied to the biomolecular sensing of a broad range of targets including the single nucleotide mutations, proteins, and cell surface receptors. 
Release date Australia
January 19th, 2019
Author
Audience
  • Professional & Vocational
Edition
Softcover reprint of the original 1st ed. 2018
Illustrations
30 Illustrations, color; 87 Illustrations, black and white; XLV, 188 p. 117 illus., 30 illus. in color.
Pages
188
ISBN-13
9789811347450
Product ID
31574122

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Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions on Hardback by Ang Yan Shan
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