The concentration of intact tissues of patients with gynecologic cancers was found of patients with epithelial ovarian cancer and in of 44% of patients with endometrial cancer. The levels were significantly higher in malignant then in non-malignant gynecologic l in tissues was found in 100% of patients with epithelial ovarian and endometrial cancers. in ovarian and uterine tumors . Different factors affecting this binding were studied and the result showed that the maximum binding was achieved by using 500 and 700 �g protein of ovarian and uterine respectively, .Association kinetic studies of the binding reaction revealed the time and temperature dependency. displaced by low concentration of unlabeled and H. The appeared that of malignant ovarian tumor have a specific -binding activity 2 fold higher than that of of benign. Endometrial cancer s have binding activity lower than that in malignant and benign ovarian tumors.Since the discovery of human hormone by ), its measurement has been the basis of pregnancy diagnosis and, a c and tumors. This hormone is synthesized by the cells of the placenta during the early weeks of pregnancy. Human progesterone until the placenta it self acquires the ability to produce this pregnancy sustaining steroid. An increasing evidence supports the synthesis of hCG in small quantities by the pituitary(62).