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In Vitro Mutagenesis Protocols

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In Vitro Mutagenesis Protocols

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In Vitro Mutagenesis Protocols by TROWER
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Description

In this volume experts from industrial and academic laboratories describe easily reproducible and up-to-date procedures for site directed and random mutagenesis. Site-directed protocols include those based on strand selection, PCR (including "splicing by overlap extension" and the "megaprimer" procedure), the ligase chain reaction, positive antibiotic selection, unique restriction site elimination, gapped heteroduplex formation, and solid-phase capture with the biotin/strepavidin system. Many can be used with virtually any double-stranded DNA plasmid. The bookcovers genesis, linker scanning mutagenesis, nested deletion mutagenesis, and a specialized E coli mutator strain.
Release date Australia
January 31st, 1996
Author
Country of Publication
United States
Illustrations
0
Imprint
Humana Press Inc.
Pages
408
Dimensions
234x156x21
ISBN-13
9780896033320
Product ID
3977968

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