The numerous advances in our ability to investigate and define cellular processes at the molecular level, have been the result of the development and application of powerful new techniques in molecular biology, in particular nucleic acid and protein methodologies. The purpose of Medical Biomethods Handbook is to introduce the reader to a wide-ranging selection of those molecular biology techniques that are most frequently used by research workers in the field of medical and clinical research.
Table of Contents
1. Basic Techniques in Molecular Biology; Ralph Rapley; 2. Probe Design, Production, and Applications; Marilena Aquino de Muro; 3. Restriction Enzymes: Tools in Clinical Research; Gareth J. S. Jenkins; 4. Southern Blotting as a Diagnostic Method; Bronwen Harvey and Pirkko Soundy; 5. Western Blotting as a Diagnostic Method; Pirkko Soundy and Bronwen Harvey; 6. Principles and Medical Applications of the Polymerase Chain Reaction; Bimal D. M. Theophilus; 7. Single Strand Conformation Polymorphism (SSCP) Analysis; Igor Vorechovsky; 8. Denaturing Gradient Gel Electrophoresis (DGGE); Jeroen H. Roelfsema and Dorien J. M. Peters; 9. Quantitative Analysis of DNA Sequences by PCR and Solid Phase Sequencing; Anu Suomalainen and Ann-Christine Syvanen; 10. Capillary Electrophoresis of DNA: Biomedical Applications; Beatriz Sanchez- Vega; 11. Mapping Techniques; Simon G. Gregory; 12. Dideoxyfingerprinting for Mutation Detection; Ioannis Bossis, Antonius Voutetakis, and Constantine A. Stratakis; 13. Conformation Sensitive Gel Electrophoresis (CSGE); Marian Hill; 14. Amplification Refractory Mutation System (ARMS) and Molecular Diagnostics; Richard Kitching and Arun Seth; 15. Ligase Chain Reaction; Carla Osiowy; 16. Chemical Cleavage of Mismatch: Theory and Clinical Applications; Neil V. Whittock and Louise Izatt; 17. The Protein Truncation Test (PTT); Johan T. den Dunnen; 18. Linkage, Allele Sharing, and Association; Mara Giordano; 19. Single Nucleotide Polymorphisms (SNPs): Technology and Applications; Cyril Mamotte, Frank Christiansen, and Lyle J. Palmer; 20. cDNA Microarrays; Philips Febbo; 21. Nucleic Acid Sequence-Based Amplification (NASBA); Katherine Loens, D. Ursi, H. Goosens, and M. Ieven; 22. The Oligonucleotide Ligation Assay; Faye A. Eggerding; 23. Quantitative TaqMan Real-Time PCR: dDagnostic and Scientific Applications; Jorg Dotsch, Ellen Schoof, and Wolfgang Rascher; 24. The Use of Denaturing High Performance Liquid Chromotography (HPLC) in Molecular Medicine; Elizabeth L. Rugg and Gareth J. Magee; 25. Quantitative PCR; David Sugden; 26. Liquid Chromatography-Mass Spectrometry of Nucleic Acids; Herbert Oberacher and Walther Parson; 27. Comparative Genomic Hybridization (CGH) in Clinical and Medical Research; Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin; 28. Bioinformatics Tools for Gene and Protein Sequence Analysis; Bernd H. A. Rehm and Frank Reinecke; 29. In Situ Hybridization; Amanda D. Watters; 30. Enzyme-Linked Immunosorbent Assay (ELISA); William J. Jordan; 31. Protein Therapeutics: Mouse, Humanized, and Human Antibodies
