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MicroRNA Profiling
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MicroRNA Profiling

Methods and Protocols
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Description

This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.
Release date Australia
June 20th, 2018
Audience
  • Professional & Vocational
Contributor
  • Edited by Sweta Rani
Edition
Softcover reprint of the original 1st ed. 2017
Illustrations
32 Illustrations, color; 9 Illustrations, black and white; XI, 246 p. 41 illus., 32 illus. in color.
Pages
246
ISBN-13
9781493982257
Product ID
28329704

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Pre-clinical medicine: basic sciences >
Medical genetics