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Optical Tweezers

Methods and Protocols
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Description

The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Optical Tweezers: Methods and Protocols aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines. 
Release date Australia
November 15th, 2016
Audience
  • Professional & Vocational
Contributor
  • Edited by Arne Gennerich
Edition
1st ed. 2017
Illustrations
133 Illustrations, color; 21 Illustrations, black and white; XII, 555 p. 154 illus., 133 illus. in color.
Pages
555
Dimensions
178x254x32
ISBN-13
9781493964192
Product ID
25447575

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