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Protein'Ligand Interactions

Methods and Applications
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Description

A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Release date Australia
March 21st, 2005
Audience
  • Professional & Vocational
Contributor
  • Edited by G. Ulrich Nienhaus
Illustrations
XI, 568 p.
Pages
568
Dimensions
155x235x36
ISBN-13
9781588293725
Product ID
2043211

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