This give information about news formulated Pakistani Honey Ointment against skin wound infection treatment. It is cheep medicine for this treatment. Result shows that it ointment give better results on human skin.The significant achieved results of honey against wound infecting bacteria are conducive and led to the development of a new honey ointment formulation. Clinical trials prove that newly formulated ointment containing 20% antimicrobial active honey is natural and significantly more effective for the treatment of skin wound infections and it provides a broader range of antimicrobial, anti-inflammatory properties and promotes wound debridement, maintains a moist wound environment, deodorize wound and stimulates healing processes.This research could be used as a reference and a baseline study for the further studies.This book is useful for the student, researchers and teacher of the Microbiology