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The Inflammasome

Methods and Protocols
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Description

 Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1β cytokines, and analysis of inflammasome assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Release date Australia
August 23rd, 2016
Audience
  • Professional & Vocational
Contributors
  • Edited by Christine M De Nardo
  • Edited by Eicke Latz
Edition
Softcover reprint of the original 1st ed. 2013
Illustrations
21 Illustrations, color; 23 Illustrations, black and white; XI, 225 p. 44 illus., 21 illus. in color.
Pages
225
Dimensions
178x254x13
ISBN-13
9781493960118
Product ID
25701717

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