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Gigaseal Formation in Patch Clamping by Majid Malboubi
Gigaseal Formation in Patch Clamping by Majid Malboubi
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Gigaseal Formation in Patch Clamping

With Applications of Nanotechnology
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Description

This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.
Release date Australia
August 7th, 2013
Audience
  • Professional & Vocational
Illustrations
65 Illustrations, color; 16 Illustrations, black and white; XI, 94 p. 81 illus., 65 illus. in color.
Pages
94
Dimensions
155x235x8
ISBN-13
9783642391279
Product ID
21447301

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